Cover of Juan Carlos Lacal (EDT), Rosario Perona (EDT), James Feramisco (EDT): Microinjection

Juan Carlos Lacal (EDT), Rosario Perona (EDT), James Feramisco (EDT) Microinjection

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Birkhauser Basel

2013

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978-3-0348-8705-2

3-0348-8705-1

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This manual describes the methodology and applications of microinjection, a powerful technique for transposing material into living cells through needle injection. Information is presented on the techniques principles, the required equipment, preparation of receiving cells and injected material, and the analysis of results. Detailed protocols for a wide range of applications in different cellular systems are included. A large part of the book is also dedicated to the Xenopus laevis oocyte system.

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